FAIRMol

KB_HAT_96

Pose ID 795 Compound 422 Pose 117

DB SELECTIONDocking pose analysis is being read from this database.
Molecular metrics status: done
RDKit SASA-based burial metrics are cached.
SASA cached
T02
Human DHFR Human
Ligand KB_HAT_96
PDB5SD8

3D complex viewer

Strict H-bonds Permissive H-bonds
Viewer legend
Protein receptor
Pocket residues
Cofactor context
Docked ligand
Overall assessment
Promising SASA cached
Promising but geometrically suspicious
Binding strong Geometry low Native strong SASA done
Strain ΔE
13.2 kcal/mol
Protein clashes
3
Internal clashes
7
Native overlap
contact recall 0.86, Jaccard 0.82, H-bond role recall 0.20
Burial
91%
Hydrophobic fit
79%
Reason: 7 internal clashes
3 protein-contact clashes 7 intramolecular clashes
Molecular report
Full metrics ↗
Promising Reasonable quality metrics. Warrants further investigation.
✓ Excellent LE (-1.023 kcal/mol/HA) ✓ Good fit quality (FQ -9.97) ✓ Good H-bonds (5 bonds) ✓ Deep burial (91% SASA buried) ✓ Lipophilic contacts well-matched (79%) ✗ Moderate strain (13.2 kcal/mol) ✗ Geometry warnings ✗ Minor protein-contact clashes (3) ✗ Internal clashes (7)
Score
-31.713
kcal/mol
LE
-1.023
kcal/mol/HA
Fit Quality
-9.97
FQ (Leeson)
HAC
31
heavy atoms
MW
414
Da
LogP
2.35
cLogP
Strain ΔE
13.2 kcal/mol
SASA buried
91%
Lipo contact
79% BSA apolar/total
SASA unbound
719 Ų
Apolar buried
516 Ų

Interaction summary

HB 5 HY 24 PI 2 CLASH 0
Final rank0.482Score-31.713
Inter norm-0.820Intra norm-0.203
Top1000noExcludedno
Contacts19H-bonds5
Artifact reasongeometry warning; 7 clashes; 3 protein contact clashes; 1 severe cofactor-context clash
Residues
ALA10 ASN65 ASP22 GLN36 GLU31 GLY21 ILE61 ILE8 LEU23 LEU68 NAP201 PHE32 PHE35 PRO27 PRO62 SER60 TYR122 VAL116 VAL9

Protein summary

200 residues
Protein targetT02Atoms3128
Residues200Chains2
Residue summaryLYS:374; LEU:361; GLU:240; VAL:224; ARG:192; PHE:180; ILE:171; PRO:168; ASN:140; SER:132; TYR:126; GLN:119; ASP:108; MET:102; THR:98; GLY:91

Receptor context

1 kept / 0 excluded
Receptor context filtering: interactions and SASA are computed against protein atoms plus allowed cofactors/ions. Native ligand-like HETATM partners are excluded from scoring.
Kept context 1 Excluded HETATM 0
Kept cofactors / ions
A:NAP201

Native ligand reference

★ reference
Interaction fingerprint calculated directly from the uploaded native ligand without docking. Current H-bond mode: strict.
Name5SD8Contacts21
PoseOpen native poseHB0
IFP residues
ALA10 ASN65 ASP22 GLN36 GLU31 GLY117 GLY21 ILE61 ILE8 LEU23 LEU68 NAP201 PHE32 PHE35 PRO62 SER60 THR137 THR57 TYR122 VAL116 VAL9
Current overlap18Native recall0.86
Jaccard0.82RMSD-
HB strict1Strict recall0.20
HB same residue+role1HB role recall0.20
HB same residue1HB residue recall0.20

HB · H-bonds

Mode: strict. Count shows atom-level H-bonds; unique residues in summary: 0.

PI · π–π interactions

Native π–π recall is disabled because no explicit native π–π reference was stored.

HY · Hydrophobic contacts

CLASH · Clashes

No clash · clashes detected for this pose.

All stored poses for this docking hit

PoseFinal rankInter normScoreHBCTCT overlapCT recallHB role rec.RMSDExcluded
117 0.4816532383386699 -0.819823 -31.7131 5 19 18 0.86 0.20 - no Current
121 0.8528228617151145 -1.04358 -33.743 5 17 0 0.00 0.00 - no Open
116 0.994354898836697 -0.86191 -27.8752 4 16 0 0.00 0.00 - no Open
88 1.6774821073098232 -1.10093 -32.8614 10 20 0 0.00 0.00 - no Open
127 2.448460325432168 -0.829026 -22.9346 4 20 19 0.90 0.40 - no Open
81 2.757958246941406 -0.758658 -26.9153 4 13 0 0.00 0.00 - no Open
86 3.5513891838815015 -0.79038 -20.7592 11 15 0 0.00 0.00 - no Open
125 3.6953505570332297 -0.629977 -25.6744 10 11 0 0.00 0.00 - no Open
49 4.429218295957923 -1.0087 -27.8555 16 18 0 0.00 0.00 - no Open
73 6.223113891236293 -1.0244 -28.8817 9 22 0 0.00 0.00 - no Open

Molecular metrics

RDKit SASA burial, strain energy (MMFF94s), ligand efficiency and fit quality for this docking pose.
✓ Metrics available

Scoring & efficiency

Docking score -31.713kcal/mol
Ligand efficiency (LE) -1.0230kcal/mol/HA
Score / heavy atom count
Fit quality (FQ) -9.966
LE / (0.072 + 0.95/HAC) — Leeson & Springthorpe
Heavy atom count 31HA

Physicochemical properties

Molecular weight 414.5Da
Lipinski: ≤ 500 Da
LogP (cLogP) 2.35
Lipinski: ≤ 5
Rotatable bonds 7

Conformational strain (MMFF94s)

Strain energy (ΔE) 13.18kcal/mol
< 5 good · 5–10 marginal · > 10 problematic
Docked FF energy -17.16kcal/mol
Minimised FF energy -30.34kcal/mol

SASA & burial

✓ computed
SASA (unbound) 719.0Ų
Total solvent-accessible surface area of free ligand
BSA total 653.6Ų
Buried surface area upon binding
BSA apolar 516.1Ų
Hydrophobic contacts buried
BSA polar 137.5Ų
Polar contacts buried
Fraction buried 90.9%
> 60 % indicates good pocket engagement
Lipophilic contact ratio 79.0%
BSA apolar / BSA total — high = hydrophobic driver
Δ Non-polar SASA -1641.3Ų
SASA_nonpolar(complex) − SASA_nonpolar(receptor) − SASA_nonpolar(ligand free). Negative = non-polar surface buried upon binding. Requires full polarity-decomposed SASA computation.
Receptor non-polar SASA 1677.9Ų
Non-polar SASA of receptor alone (VdW proxy, nonpolar atoms only)
Complex non-polar SASA 612.2Ų
Non-polar SASA of full complex (VdW proxy, nonpolar atoms only)