FAIRMol

Z56575886

Pose ID 5994 Compound 724 Pose 576

DB SELECTIONDocking pose analysis is being read from this database.
Molecular metrics status: done
RDKit SASA-based burial metrics are cached.
SASA cached
T09
L. major DHFR-TS L. major
Ligand Z56575886

3D complex viewer

Strict H-bonds Permissive H-bonds
Viewer legend
Protein receptor
Pocket residues
Cofactor context
Docked ligand
Overall assessment
Reject SASA cached
Promising but geometrically suspicious
Binding strong Geometry low Native strong SASA done
Strain ΔE
43.9 kcal/mol
Protein clashes
4
Internal clashes
4
Native overlap
contact recall 0.57, Jaccard 0.46, H-bond role recall 0.00
Burial
84%
Hydrophobic fit
79%
Reason: strain 43.9 kcal/mol
strain ΔE 43.9 kcal/mol 4 protein-contact clashes 4 intramolecular clashes
EcoTox / ADMET In-silico prediction only
Fish LC₅₀
Cat. 1 — Very toxic (≤1 mg/L)
Daphnia EC₅₀
Cat. 1 — Very toxic (≤1 mg/L)
Algae IC₅₀
Cat. 1 — Very toxic (≤1 mg/L)
Bioaccum.
B — Bioaccumulative (BCF>2000)
Persistent
Yes
ADMET alerts (in-silico)
hERG High Ames Clear DILI Risk
Molecular report
Full metrics ↗
Reject Multiple quality flags — this pose should be deprioritised or discarded.
✓ Excellent LE (-0.768 kcal/mol/HA) ✓ Good fit quality (FQ -7.55) ✓ Deep burial (84% SASA buried) ✓ Lipophilic contacts well-matched (79%) ✗ Extreme strain energy (43.9 kcal/mol) ✗ Geometry warnings ✗ Protein-contact clashes (5) ✗ Many internal clashes (13)
Score
-24.563
kcal/mol
LE
-0.768
kcal/mol/HA
Fit Quality
-7.55
FQ (Leeson)
HAC
32
heavy atoms
MW
483
Da
LogP
6.13
cLogP
Final rank
1.3891
rank score
Inter norm
-0.878
normalised
Contacts
17
H-bonds 3
Strain ΔE
43.9 kcal/mol
SASA buried
84%
Lipo contact
79% BSA apolar/total
SASA unbound
725 Ų
Apolar buried
484 Ų

Interaction summary

HBA 1 HY 11 PI 2 CLASH 4

HBD/HBA · H-bonds (geometric)

HBD = ligand donates H · HBA = ligand accepts H · ~ = weak (≥110°). Mode: strict. Residues: 1.

PI · π–π interactions

Native π–π recall is disabled because no explicit native π–π reference was stored.

HY · Hydrophobic contacts

CLASH · Clashes

Native ligand reference

★ reference
Interaction fingerprint calculated directly from the uploaded native ligand without docking. Current H-bond mode: strict.
Name3CL9Contacts21
PoseOpen native poseHB0
IFP residues
NDP301 ALA32 ARG97 ASP52 GLY157 ILE45 LEU94 LYS57 MET53 PHE55 PHE56 PHE91 PRO88 SER86 THR180 THR83 TYR162 VAL156 VAL30 VAL31 VAL87
Current overlap12Native recall0.57
Jaccard0.46RMSD-
HB strict0Strict recall0.00
HB same residue+role0HB role recall0.00
HB same residue0HB residue recall0.00

Protein summary

511 residues
Protein targetT09Atoms8170
Residues511Chains2
Residue summaryLEU:1064; ARG:840; LYS:572; GLU:570; VAL:528; PHE:460; ILE:456; ALA:440; PRO:420; THR:406; TYR:357; ASP:312; GLN:289; ASN:238; SER:231; GLY:224

Receptor context

1 kept / 0 excluded
Receptor context filtering: interactions and SASA are computed against protein atoms plus allowed cofactors/ions. Native ligand-like HETATM partners are excluded from scoring.
Kept context 1 Excluded HETATM 0
Kept cofactors / ions
A:NDP301

All stored poses for this docking hit

PoseFinal rankInter normScoreHBCTCT overlapCT recallHB role rec.RMSDExcluded
568 0.849337216587434 -0.939978 -28.0315 2 17 0 0.00 0.00 - no Open
584 0.9532260108307988 -0.899914 -23.4592 1 20 0 0.00 0.00 - no Open
576 1.3890939222123488 -0.877617 -24.5626 3 17 12 0.57 0.00 - no Current
602 3.56512257101324 -0.697649 -17.0795 2 18 0 0.00 0.00 - no Open

Molecular metrics

RDKit SASA burial, strain energy (MMFF94s), ligand efficiency and fit quality for this docking pose.
✓ Metrics available

Scoring & efficiency

Docking score -24.563kcal/mol
Ligand efficiency (LE) -0.7676kcal/mol/HA
Score / heavy atom count
Fit quality (FQ) -7.548
LE / (0.072 + 0.95/HAC) — Leeson & Springthorpe
Heavy atom count 32HA

Physicochemical properties

Molecular weight 483.0Da
Lipinski: ≤ 500 Da
LogP (cLogP) 6.13
Lipinski: ≤ 5
Rotatable bonds 6

Conformational strain (MMFF94s)

Strain energy (ΔE) 43.91kcal/mol
< 5 good · 5–10 marginal · > 10 problematic
Docked FF energy 71.38kcal/mol
Minimised FF energy 27.47kcal/mol

SASA & burial

✓ computed
SASA (unbound) 724.9Ų
Total solvent-accessible surface area of free ligand
BSA total 609.5Ų
Buried surface area upon binding
BSA apolar 484.2Ų
Hydrophobic contacts buried
BSA polar 125.3Ų
Polar contacts buried
Fraction buried 84.1%
> 60 % indicates good pocket engagement
Lipophilic contact ratio 79.4%
BSA apolar / BSA total — high = hydrophobic driver
Δ Non-polar SASA -3310.6Ų
SASA_nonpolar(complex) − SASA_nonpolar(receptor) − SASA_nonpolar(ligand free). Negative = non-polar surface buried upon binding. Requires full polarity-decomposed SASA computation.
Receptor non-polar SASA 4428.5Ų
Non-polar SASA of receptor alone (VdW proxy, nonpolar atoms only)
Complex non-polar SASA 1716.1Ų
Non-polar SASA of full complex (VdW proxy, nonpolar atoms only)