FAIRMol

MK220

Pose ID 5667 Compound 167 Pose 249

DB SELECTIONDocking pose analysis is being read from this database.
Molecular metrics status: done
RDKit SASA-based burial metrics are cached.
SASA cached
T09
L. major DHFR-TS L. major
Ligand MK220

3D complex viewer

Strict H-bonds Permissive H-bonds
Viewer legend
Protein receptor
Pocket residues
Cofactor context
Docked ligand
Overall assessment
Weak SASA cached
Promising but geometrically suspicious
Binding strong Geometry low Native strong SASA done
Strain ΔE
22.2 kcal/mol
Protein clashes
0
Internal clashes
12
Native overlap
contact recall 0.71, Jaccard 0.60, H-bond role recall 0.17
Burial
84%
Hydrophobic fit
82%
Reason: 12 internal clashes
12 intramolecular clashes
Molecular report
Full metrics ↗
Weak Marginal quality. Consider only alongside better-scoring alternatives.
✓ Excellent LE (-0.628 kcal/mol/HA) ✓ Good fit quality (FQ -6.18) ✓ Good H-bonds (3 bonds) ✓ Deep burial (84% SASA buried) ✓ Lipophilic contacts well-matched (82%) ✗ High strain energy (22.2 kcal/mol) ✗ Geometry warnings ✗ Many internal clashes (12)
Score
-20.106
kcal/mol
LE
-0.628
kcal/mol/HA
Fit Quality
-6.18
FQ (Leeson)
HAC
32
heavy atoms
MW
438
Da
LogP
4.28
cLogP
Strain ΔE
22.2 kcal/mol
SASA buried
84%
Lipo contact
82% BSA apolar/total
SASA unbound
726 Ų
Apolar buried
497 Ų

Interaction summary

HB 3 HY 24 PI 2 CLASH 0
Final rank3.165Score-20.106
Inter norm-0.802Intra norm0.174
Top1000noExcludedno
Contacts19H-bonds3
Artifact reasongeometry warning; 12 clashes; 2 protein clashes; moderate strain Δ 22.2
Residues
NDP301 ALA32 ARG48 ASP52 GLY157 ILE45 MET53 PHE56 PHE91 PRO50 PRO88 THR83 TRP47 TYR162 VAL156 VAL30 VAL31 VAL49 VAL87

Protein summary

511 residues
Protein targetT09Atoms8170
Residues511Chains2
Residue summaryLEU:1064; ARG:840; LYS:572; GLU:570; VAL:528; PHE:460; ILE:456; ALA:440; PRO:420; THR:406; TYR:357; ASP:312; GLN:289; ASN:238; SER:231; GLY:224

Receptor context

1 kept / 0 excluded
Receptor context filtering: interactions and SASA are computed against protein atoms plus allowed cofactors/ions. Native ligand-like HETATM partners are excluded from scoring.
Kept context 1 Excluded HETATM 0
Kept cofactors / ions
A:NDP301

Native ligand reference

★ reference
Interaction fingerprint calculated directly from the uploaded native ligand without docking. Current H-bond mode: strict.
Name3CL9Contacts21
PoseOpen native poseHB0
IFP residues
NDP301 ALA32 ARG97 ASP52 GLY157 ILE45 LEU94 LYS57 MET53 PHE55 PHE56 PHE91 PRO88 SER86 THR180 THR83 TYR162 VAL156 VAL30 VAL31 VAL87
Current overlap15Native recall0.71
Jaccard0.60RMSD-
HB strict1Strict recall0.14
HB same residue+role1HB role recall0.17
HB same residue1HB residue recall0.17

HB · H-bonds

Mode: strict. Count shows atom-level H-bonds; unique residues in summary: 0.

PI · π–π interactions

Native π–π recall is disabled because no explicit native π–π reference was stored.

HY · Hydrophobic contacts

CLASH · Clashes

No clash · clashes detected for this pose.

All stored poses for this docking hit

PoseFinal rankInter normScoreHBCTCT overlapCT recallHB role rec.RMSDExcluded
340 1.3223938073115098 -0.927161 -24.2651 8 16 0 0.00 0.00 - no Open
394 2.014161173473132 -0.775497 -19.6976 10 10 0 0.00 0.00 - no Open
290 2.73482945957276 -0.878682 -21.3656 12 17 0 0.00 0.00 - no Open
249 3.165423711787248 -0.802147 -20.1063 3 19 15 0.71 0.17 - no Current
314 3.7162080227370713 -0.874068 -20.3152 7 13 0 0.00 0.00 - no Open
365 3.9636524130908617 -0.716261 -19.4033 6 14 0 0.00 0.00 - no Open
217 4.166365624759972 -0.935886 -25.7232 10 16 0 0.00 0.00 - no Open
330 4.277927265958028 -0.950834 -24.4595 5 17 1 0.05 0.00 - no Open
321 4.96208626260307 -0.705542 -18.3404 7 11 0 0.00 0.00 - no Open
241 5.4952799348659935 -0.968125 -29.9385 9 25 0 0.00 0.00 - no Open

Molecular metrics

RDKit SASA burial, strain energy (MMFF94s), ligand efficiency and fit quality for this docking pose.
✓ Metrics available

Scoring & efficiency

Docking score -20.106kcal/mol
Ligand efficiency (LE) -0.6283kcal/mol/HA
Score / heavy atom count
Fit quality (FQ) -6.179
LE / (0.072 + 0.95/HAC) — Leeson & Springthorpe
Heavy atom count 32HA

Physicochemical properties

Molecular weight 437.5Da
Lipinski: ≤ 500 Da
LogP (cLogP) 4.28
Lipinski: ≤ 5
Rotatable bonds 9

Conformational strain (MMFF94s)

Strain energy (ΔE) 22.16kcal/mol
< 5 good · 5–10 marginal · > 10 problematic
Docked FF energy 37.94kcal/mol
Minimised FF energy 15.77kcal/mol

SASA & burial

✓ computed
SASA (unbound) 725.5Ų
Total solvent-accessible surface area of free ligand
BSA total 605.6Ų
Buried surface area upon binding
BSA apolar 496.7Ų
Hydrophobic contacts buried
BSA polar 108.9Ų
Polar contacts buried
Fraction buried 83.5%
> 60 % indicates good pocket engagement
Lipophilic contact ratio 82.0%
BSA apolar / BSA total — high = hydrophobic driver
Δ Non-polar SASA -3376.8Ų
SASA_nonpolar(complex) − SASA_nonpolar(receptor) − SASA_nonpolar(ligand free). Negative = non-polar surface buried upon binding. Requires full polarity-decomposed SASA computation.
Receptor non-polar SASA 4428.5Ų
Non-polar SASA of receptor alone (VdW proxy, nonpolar atoms only)
Complex non-polar SASA 1653.9Ų
Non-polar SASA of full complex (VdW proxy, nonpolar atoms only)